酸(suan)值(zhi)試驗儀的試驗步驟∶

1、驅除二氧化碳取95%的(de)乙醇(chun)50毫升注入清潔無水的(de)錐形燒瓶內(nei)，用裝(zhuang)有(you)回流冷(leng)凝管的(de)軟(ruan)木塞塞住錐形燒瓶后，將95%的(de)乙醇(chun)煮沸5分鐘。

2、中(zhong)(zhong)和抽提溶(rong)劑(ji)﹐在(zai)煮(zhu)沸過(guo)的(de)95%的(de)乙醇中(zhong)(zhong)加入0.5毫升(sheng)的(de)堿性(xing)藍溶(rong)液(ye)(ye)（或酚酞(tai)溶(rong)液(ye)(ye))后(hou)，在(zai)不(bu)斷搖蕩下趁熱用(yong)0.05M的(de)氫氧(yang)化(hua)鉀乙醇溶(rong)液(ye)(ye)滴(di)(di)定，直(zhi)至(zhi)錐形瓶中(zhong)(zhong)混合物的(de)顏色由藍色變為淺紅(hong)色或滴(di)(di)至(zhi)淺玫瑰(gui)紅(hong)色為止(zhi)。

3、取(qu)樣(yang)―汽(qi)油或煤(mei)油取(qu)50 mL，柴油取(qu)20 mL(均在20℃±3℃溫度范圍(wei)內量取(qu))將試(shi)樣(yang)注入(ru)中(zhong)和過的熱的95%乙醇中(zhong)。

4、滴(di)定(ding)操(cao)作將(jiang)球形(xing)回流冷凝管裝(zhuang)到錐形(xing)瓶(ping)上之后，將(jiang)錐形(xing)瓶(ping)中(zhong)的混(hun)(hun)合物(wu)煮沸5 min，對已加(jia)(jia)(jia)有(you)堿(jian)性(xing)藍(lan)6B溶(rong)(rong)(rong)液(ye)(ye)或甲(jia)酚(fen)(fen)(fen)紅(hong)溶(rong)(rong)(rong)液(ye)(ye)的混(hun)(hun)合物(wu)，此時應再(zai)加(jia)(jia)(jia)入0.5 mL的堿(jian)性(xing)藍(lan)6B溶(rong)(rong)(rong)液(ye)(ye)或甲(jia)酚(fen)(fen)(fen)紅(hong)溶(rong)(rong)(rong)液(ye)(ye)，仍需在不斷搖(yao)蕩下(xia)趁(chen)熱用0.05 mol / L氫氧化鉀乙醇溶(rong)(rong)(rong)液(ye)(ye)滴(di)定(ding)，直至95%乙醇層的堿(jian)性(xing)藍(lan)6B溶(rong)(rong)(rong)液(ye)(ye)從藍(lan)色變(bian)為淺紅(hong)色(甲(jia)酚(fen)(fen)(fen)紅(hong)溶(rong)(rong)(rong)液(ye)(ye)從黃色變(bian)為紫紅(hong)色)為止(zhi);或對已加(jia)(jia)(jia)有(you)酚(fen)(fen)(fen)酞(tai)溶(rong)(rong)(rong)液(ye)(ye)的混(hun)(hun)合物(wu)，此時應再(zai)加(jia)(jia)(jia)入1~②滴(di)酚(fen)(fen)(fen)酞(tai)溶(rong)(rong)(rong)液(ye)(ye)，按上述操(cao)作直至95%乙醇層的酚(fen)(fen)(fen)酞(tai)溶(rong)(rong)(rong)液(ye)(ye)呈現淺玫瑰紅(hong)色為止(zhi)。